Human N (6) -adenine-specific DNA methyltransferase 2 (N6AMT2) ELISA Kit

EEF1AKMT1 (Eukaryotic Translation Elongation Factor 1 Alpha Lysine Methyltransferase 1) is a Protein Coding gene. Among its related pathways are Metabolism of proteins. S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha.

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Human N (6) -adenine-specific DNA methyltransferase 2 (N6AMT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human N6AMT2. No significant cross-reactivity or interference between Human N6AMT2 and analogues was observed.

This Human N (6) -adenine-specific DNA methyltransferase 2 (N6AMT2) ELISA Kit employs a two-site sandwich ELISA to quantitate N6AMT2 in samples. An antibody specific for N6AMT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyN6AMT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for N6AMT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of N6AMT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.