Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit

NEDD8-activating enzyme E1 regulatory subunit encoded by NAE1 binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for NAE1.

NAE1; A-116A10.1; APPBP1; HPP1; ula-1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein binding protein 1; 59kDa; amyloid beta precursor protein-binding protein 1; 59kD; amyloid protein-bin

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Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAE1. No significant cross-reactivity or interference between Human NAE1 and analogues was observed.

This Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAE1 in samples. An antibody specific for NAE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NAE1; A-116A10.1; APPBP1; HPP1; ula-1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein binding protein 1; 59kDa; amyloid beta precursor protein-binding protein 1; 59kD; amyloid protein-bin

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.