Human Sodium leak channel non-selective protein (NALCN) ELISA Kit

NALCN encodes a voltage-independent, nonselective cation channel which belongs to a family of voltage-gated sodium and calcium channels that regulates the resting membrane potential and excitability of neurons. This family is expressed throughout the nervous system and conducts a persistent sodium leak current that contributes to tonic neuronal excitability. Sodium leak channel non-selective protein forms a channelosome complex that includes G-protein-coupled receptors, UNC-79, UNC-80, NCA localization factor-1, and src family tyrosine kinases. Naturally occurring mutations in NALCN are associated with infantile neuroaxonal dystrophy, infantile hypotonia with psychomotor retardation and characteristic facies (IHPRF) syndrome, and congenital contractures of the limbs and face with hypotonia and developmental delay (CLIFAHDD) syndrome. A knockout of the orthologous gene in mice results in paralysis with a severely disrupted respiratory rhythm, and lethality within 24 hours after birth.

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Human Sodium leak channel non-selective protein (NALCN) ELISA Kit has high sensitivity and excellent specificity for detection of Human NALCN. No significant cross-reactivity or interference between Human NALCN and analogues was observed.

This Human Sodium leak channel non-selective protein (NALCN) ELISA Kit employs a two-site sandwich ELISA to quantitate NALCN in samples. An antibody specific for NALCN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNALCN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NALCN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NALCN bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.