Human Probable asparaginyl-tRNA synthetase, mitochondrial (NARS2) ELISA Kit

NARS2 encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in NARS2 have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24) .

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Human Probable asparaginyl-tRNA synthetase, mitochondrial (NARS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human NARS2. No significant cross-reactivity or interference between Human NARS2 and analogues was observed.

This Human Probable asparaginyl-tRNA synthetase, mitochondrial (NARS2) ELISA Kit employs a two-site sandwich ELISA to quantitate NARS2 in samples. An antibody specific for NARS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNARS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NARS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NARS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.