Human Probable N-acetyltransferase 8B (NAT8B) ELISA Kit

Probable N-acetyltransferase 8B encoded by NAT8B is highly similar to the N-acetyltransferase 8 (NAT8) gene product, which is a kidney and liver protein with homology to bacterial acetyltransferases involved in drug resistance. NAT8B is localized on chromosome 2 in the vicinity of the NAT8 gene and may represent a pseudogene of NAT8. NAT8B contains two polymorphic nonsense mutations that disrupt the active site of the protein. The full-length product of NAT8B contains a complete acetyltransferase domain and is identical in length to NAT8.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Probable N-acetyltransferase 8B (NAT8B) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAT8B. No significant cross-reactivity or interference between Human NAT8B and analogues was observed.

This Human Probable N-acetyltransferase 8B (NAT8B) ELISA Kit employs a two-site sandwich ELISA to quantitate NAT8B in samples. An antibody specific for NAT8B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAT8B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT8B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT8B bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.