Human Next to BRCA1 gene 2 protein (NBR2) ELISA Kit

NBR2 (Neighbor Of BRCA1 Gene 2 (Non-Protein Coding) ) is an RNA Gene, and is affiliated with the non-coding RNA class. NBR2 was identified by its close proximity on chromosome 17 to tumor suppressor gene BRCA1. Experimental evidence indicates that the two genes share a bi-directional promoter. Transcription for either gene is controlled individually by distinct transcriptional repressor factors. A short (112 amino acid) open reading frame is observed which includes a region derived from a LINE1 element. A strong Kozak signal is not observed for the putative ORF and the stop codon is more than 55 nucleotides upstream of the last splice site for the transcript, suggesting that the transcript is subject to nonsense-mediated decay. Therefore, this gene does not appear to encode a protein. Glucose starvation induces the expression of this gene and the long non-coding RNA transcribed by it functions with AMP-activated protein kinase in mediating the energy stress response.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Next to BRCA1 gene 2 protein (NBR2) ELISA Kit has high sensitivity and excellent specificity for detection of Human NBR2. No significant cross-reactivity or interference between Human NBR2 and analogues was observed.

This Human Next to BRCA1 gene 2 protein (NBR2) ELISA Kit employs a two-site sandwich ELISA to quantitate NBR2 in samples. An antibody specific for NBR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNBR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NBR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NBR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.