Human Nuclear receptor coactivator 4 (NCOA4) ELISA Kit

This Human Nuclear receptor coactivator 4 (NCOA4) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA4.

Product Specifications

Background

NCOA4 encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between NCOA4 and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.

CAS Number

7732-18-5

Product Name Alternative

NCOA4; RP11-481A12.4; ARA70; DKFZp762E1112; ELE1; PTC3; RFG; RET-activating gene ELE1; androgen receptor coactivator; 70-kD; papillary thyroid carcinoma 3; ret fused

Gene ID

8031

Accession Number

Q13772

Reactivity

Human

Tag

NCOA4

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

Please inquire

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Human Nuclear receptor coactivator 4 (NCOA4) ELISA Kit has high sensitivity and excellent specificity for detection of Human NCOA4. No significant cross-reactivity or interference between Human NCOA4 and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

This Human Nuclear receptor coactivator 4 (NCOA4) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA4 in samples. An antibody specific for NCOA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

NCOA4; RP11-481A12.4; ARA70; DKFZp762E1112; ELE1; PTC3; RFG; RET-activating gene ELE1; androgen receptor coactivator; 70-kD; papillary thyroid carcinoma 3; ret fused

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.