Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. The beta subunit (P4HB) is an unusual multifunctional polypeptide identical to protein disulfide isomerase. P4HA2 is one of at least 2 alpha subunit isoforms. This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains.

P4HA2; 4-PH alpha 2; C-P4Halpha (II) ; OTTHUMP00000065966; OTTHUMP00000065969; collagen prolyl 4-hydroxylase alpha (II) ; procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase) ; alpha polypepti

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Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human P4HA2. No significant cross-reactivity or interference between Human P4HA2 and analogues was observed.

This Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA2 in samples. An antibody specific for P4HA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

P4HA2; 4-PH alpha 2; C-P4Halpha (II) ; OTTHUMP00000065966; OTTHUMP00000065969; collagen prolyl 4-hydroxylase alpha (II) ; procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase) ; alpha polypepti

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.