Human Pantothenate kinase 4 (PANK4) ELISA Kit

Pantothenate kinase 4 is a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is most abundant in muscle but is expressed in all tissues.Pantothenate kinase is an essential regulatory enzyme in CoA biosynthesis, catalyzing the cytosolic phosphorylation of pantothenate (vitamin B5), N-pantothenoylcysteine, and pantetheine. PANK4 expression is most abundant in muscle, but it is expressed in all tissues.

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Human Pantothenate kinase 4 (PANK4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PANK4. No significant cross-reactivity or interference between Human PANK4 and analogues was observed.

This Human Pantothenate kinase 4 (PANK4) ELISA Kit employs a two-site sandwich ELISA to quantitate PANK4 in samples. An antibody specific for PANK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPANK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PANK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PANK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.