Human Paired box protein Pax-9 (PAX9) ELISA Kit

PAX9 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 9 gene is unknown but it may involve development of stratified squamous epithelia as well as various organs and skeletal elements.Stapleton et al. (1993) isolated a cosmid for PAX9, a novel member of the paired box-containing gene family. They found it to be closely related in its paired domain to PAX1. Wallin et al. (1993) independently cloned the Pax9 gene in the mouse. PAX9 is a member of the same subgroup as Pax1/undulated.

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Human Paired box protein Pax-9 (PAX9) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAX9. No significant cross-reactivity or interference between Human PAX9 and analogues was observed.

This Human Paired box protein Pax-9 (PAX9) ELISA Kit employs a two-site sandwich ELISA to quantitate PAX9 in samples. An antibody specific for PAX9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAX9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAX9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAX9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.