Human Protocadherin-20 (PCDH20) ELISA Kit

Protocadherin-20 is a protein belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain. The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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Human Protocadherin-20 (PCDH20) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCDH20. No significant cross-reactivity or interference between Human PCDH20 and analogues was observed.

This Human Protocadherin-20 (PCDH20) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDH20 in samples. An antibody specific for PCDH20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCDH20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCDH20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDH20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.