Human Neuroendocrine convertase 1 (PCSK1) ELISA Kit

Proprotein Convertase Subtilisin/Kexin Type 1 is aprotein belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity. This encoded protein is associated with carcinoid tumors. The use of alternate polyadenylation sites has been found for this gene. Multiple alternatively spliced transcript variants have been described for this gene but their full length nature is not known.

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Human Neuroendocrine convertase 1 (PCSK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCSK1. No significant cross-reactivity or interference between Human PCSK1 and analogues was observed.

This Human Neuroendocrine convertase 1 (PCSK1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCSK1 in samples. An antibody specific for PCSK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.