Human Myomegalin (PDE4DIP) ELISA Kit

By analyzing ESTs for cardiac muscle-specific transcripts, followed by screening heart cDNA libraries and 5-prime RACE, Soejima et al. (2001) cloned 4 splice variants of PDE4DIP, which they called MMGL. The variant-1 transcript has a long 3-prime untranslated region containing 9 possible polyadenylation signals, and it encodes a deduced 174-amino acid protein. Variants 2, 3, and 4 encode proteins of 174, 173, and 240 amino acids, respectively. Northern blot analysis detected high expression of a 1.0- to 1.3-kb band and lower expression of 4.4- and 8.5-kb bands in adult and fetal heart, skeletal muscle, and all specific heart regions examined. Only the 4.4-kb transcript was detected in aorta. Fluorescence-tagged variants 1 and 4 were expressed in both the cytoplasm and nucleus of transfected mouse myocytes.

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Human Myomegalin (PDE4DIP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDE4DIP. No significant cross-reactivity or interference between Human PDE4DIP and analogues was observed.

This Human Myomegalin (PDE4DIP) ELISA Kit employs a two-site sandwich ELISA to quantitate PDE4DIP in samples. An antibody specific for PDE4DIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDE4DIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDE4DIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDE4DIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.