Human Platelet-Derived Growth Factor AA (PDGF-AA) ELISA Kit

The receptor for PDGF, PDGFR is classified as a receptor tyrosine kinase (RTK), a type of cell surface receptor. Two types of PDGFRs have been identified: alpha type and beta type PDGFRs. The alpha type binds to PDGF-AA, PDGF-BB and PDGF-AB while the beta type PDGFR binds with high affinity to PDGF-BB and PDGF-AB. PDGF binds to PDGFRs ligand binding pocket located within the second and third immunoglobulin domains. Upon activation by PDGF, these receptors dimerise, and are "switched on" by auto-phosphorylation of several sites on their cytosolic domains, which serve to mediate binding of cofactors and subsequently activate signal transduction, for example, through the PI3K pathway. The role of PI3K has been investigated by several laboratories. Accumulating data suggests that while this molecule is generally part of growth signaling complex, it plays a more profound role in controlling cell migration.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Platelet-Derived Growth Factor AA (PDGF-AA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDGF-AA. No significant cross-reactivity or interference between Human PDGF-AA and analogues was observed.

This Human Platelet-Derived Growth Factor AA (PDGF-AA) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGF-AA in samples. An antibody specific for PDGF-AA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGF-AA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-AA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-AA bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.