Human Protein disulfide-isomerase A3 (PDIA3) ELISA Kit

The PDIA3 protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. The cDNA encoding human GRP58 was cloned independently by Bourdi et al. (1995), Koivunen et al. (1996), and Hirano et al. (1995) . All reported that the gene encodes a 505-amino acid polypeptide with significant homology to human protein disulfide isomerase (PDI) . Bourdi et al. (1995) noted that the sequence includes a putative nuclear localization motif and an endoplasmic reticulum (ER) -retention/retrieval motif.

PDIA3; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HsT17083; P58; PI-PLC; 58 kDa microsomal protein; endoplasmic reticulum P58; glucose regulated protein; 58kDa; phospholipase C-alpha; protein disulfide isomerase

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Protein disulfide-isomerase A3 (PDIA3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDIA3. No significant cross-reactivity or interference between Human PDIA3 and analogues was observed.

This Human Protein disulfide-isomerase A3 (PDIA3) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA3 in samples. An antibody specific for PDIA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDIA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

PDIA3; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HsT17083; P58; PI-PLC; 58 kDa microsomal protein; endoplasmic reticulum P58; glucose regulated protein; 58kDa; phospholipase C-alpha; protein disulfide isomerase

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.