Human PDZ and LIM domain protein 1 (PDLIM1) ELISA Kit

The LIM domain of PDLIM1 interacts with the C-terminal EF-hand region of alpha-actinin-2 (ACTN2) . Immunoprecipitation, Western blot analysis, and immunofluorescence microscopy demonstrated that the 36-kD PDLIM1 protein colocalizes with ACTN2 in the Z discs of myocardial sarcomeres, particularly at intercalated discs, and with vinculin (VCL), which is localized in the fascia adherens of intercalated discs. Sequence analysis predicted that the 329-amino acid PDLIM1 protein, which is 88% homologous to the rat protein, shares 51 to 61% identity with the PDZ domains of ENIGMA, ALP, ENH, and RIL. SDS-PAGE analysis showed that recombinant PDLIM1 was expressed as a 36-kD protein.

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Human PDZ and LIM domain protein 1 (PDLIM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDLIM1. No significant cross-reactivity or interference between Human PDLIM1 and analogues was observed.

This Human PDZ and LIM domain protein 1 (PDLIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PDLIM1 in samples. An antibody specific for PDLIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDLIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDLIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDLIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.