Human Phosphoglycerate kinase 1 (PGK1) ELISA Kit

The Phosphoglycerate Kinase 1 is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19.PGK1 is distinguished from testicular PGK2, which maps to chromosome 6p21. The deduced protein contains 417 amino acid residues. Southern blot analysis of human genomic DNAs showed a complex pattern of hybridizing fragments, 2 of which were non-X in origin. The results were interpreted as reflecting the existence of a small family of dispersed PGK or PGK-like genes.

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Human Phosphoglycerate kinase 1 (PGK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PGK1. No significant cross-reactivity or interference between Human PGK1 and analogues was observed.

This Human Phosphoglycerate kinase 1 (PGK1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGK1 in samples. An antibody specific for PGK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.