What is the catalog number for Human Peptidase inhibitor 16 (PI16) ELISA Kit?

The catalog number for Human Peptidase inhibitor 16 (PI16) ELISA Kit is KTE61219.

What sizes are available for Human Peptidase inhibitor 16 (PI16) ELISA Kit?

Human Peptidase inhibitor 16 (PI16) ELISA Kit is available in the following size(s): 48 Tests, 96 Tests, 5x 96 Tests, 50x 96 Tests.

How many pack sizes does Human Peptidase inhibitor 16 (PI16) ELISA Kit come in?

Human Peptidase inhibitor 16 (PI16) ELISA Kit is available in 4 different pack sizes.

Is Human Peptidase inhibitor 16 (PI16) ELISA Kit in stock?

Human Peptidase inhibitor 16 (PI16) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

Human Peptidase inhibitor 16 (PI16) ELISA Kit

This Human Peptidase inhibitor 16 (PI16) ELISA Kit employs a two-site sandwich ELISA to quantitate PI16.

Product Specifications

Background

PI16 is a putative serine protease inhibitor. PI16 may serve as a marker following prostatectomy for prostate cancer. This protein binds with high affinity to PSP94. PI16 expression is enhanced after serum stimulation of primary cardiomyocytes and is rapidly secreted into the conditioned medium of cardiomyocytes. PI16 is overexpressed in the hypertrophic and failing heart and inhibits cardiomyocyte growth. Suppression of endogenous PI16 expression in primary cardiomyocytes significantly enhances cardiomyocyte size. Transgenic mice overexpressing PI16 in a cardiomyocyte-specific manner show normal cardiac function but have smaller hearts with hypotrophic cardiomyocytes.

CAS Number

7732-18-5

Product Name Alternative

PI16; CRISP9; DKFZp586B1817; MGC45378; MSMBBP; PSPBP; PSP94-binding protein; microseminoprotein; beta-binding protein; protease inhibitor 16

Gene ID

221476

Accession Number

Q6UXB8

Reactivity

Human

Tag

PI16

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

Please inquire

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Human Peptidase inhibitor 16 (PI16) ELISA Kit has high sensitivity and excellent specificity for detection of Human PI16. No significant cross-reactivity or interference between Human PI16 and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

This Human Peptidase inhibitor 16 (PI16) ELISA Kit employs a two-site sandwich ELISA to quantitate PI16 in samples. An antibody specific for PI16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPI16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

PI16; CRISP9; DKFZp586B1817; MGC45378; MSMBBP; PSPBP; PSP94-binding protein; microseminoprotein; beta-binding protein; protease inhibitor 16

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.