Human Progesterone induced blocking factor (PIBF) ELISA Kit

PIBF encodes a predicted hydrophilic 757-amino acid alpha-helical protein with an N-terminal signal sequence, a nuclear localization signal, a leucine zipper motif, a basic zipper sequence, a PEST sequence, an endoplasmic reticulum membrane retention signal, and several potential N-glycosylation and phosphorylation sites. Immunoblot analysis, immunocytochemistry, and fluorescence microscopy of insulin-treated mammary carcinoma cells detected expression of a 34-kD secreted protein in cytoplasmic granules. In untreated cells, nucleus-associated expression of the full-length 90-kD protein was detected.PIBF1 contains 758 amino acids. multiple PIBF1 transcripts expressed at highest levels in testis. Moderate expression was detected in spleen, thymus, prostate, ovary, small intestine, and colon.

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Human Progesterone induced blocking factor (PIBF) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIBF1. No significant cross-reactivity or interference between Human PIBF1 and analogues was observed.

This Human Progesterone induced blocking factor (PIBF) ELISA Kit employs a two-site sandwich ELISA to quantitate PIBF1 in samples. An antibody specific for PIBF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIBF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIBF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIBF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.