Human Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) ELISA Kit

PIGF encodes 1 of the enzymes involved in glycosylphosphatidylinositol (GPI) anchor biosynthesis. The primary biochemical defect in paroxysmal nocturnal hemoglobinuria (PNH), an acquired hematologic disorder with protean clinical manifestations including recurrent episodes of hemolysis, infections, and venous thrombosis, involves defective synthesis of GPI anchors that attach certain proteins to the cell surface membrane. Proteins with appropriate C-terminal signal peptides are transferred to the preformed glycolipid anchor in the endoplasmic reticulum and transported to the cell surface as a single unit; in PNH, there is a defect in GPI-anchor biosynthesis and an absence of all surface proteins that use this anchoring motif.

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Human Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIGF. No significant cross-reactivity or interference between Human PIGF and analogues was observed.

This Human Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) ELISA Kit employs a two-site sandwich ELISA to quantitate PIGF in samples. An antibody specific for PIGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.