Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit

CNK is a member of the 'polo' family of serine/threonine kinases. Members of this family have been implicated in cell division and are important in regulating the onset of mitosis and M-phase progression.The predicted 607-amino acid protein has an N-terminal catalytic domain, including an ATP-binding site, a central putative nuclear targeting signal, and a presumed C-terminal regulatory domain. The CNK protein shows homology with several other members of the 'polo' family; it is 91% identical to mouse Fnk, 50% identical to human PLK, 48% identical to Drosophila polo, and 38% identical to S. cerevisiae Cdc5. Northern blot analysis detected expression of a 2.5-kb CNK transcript in a limited number of human tissues, with placenta containing a moderate level and ovary, lung, and peripheral blood leukocytes containing low levels.

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Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLK3. No significant cross-reactivity or interference between Human PLK3 and analogues was observed.

This Human Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate PLK3 in samples. An antibody specific for PLK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.