Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit

PRDX3 encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.

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Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRDX3. No significant cross-reactivity or interference between Human PRDX3 and analogues was observed.

This Human Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX3 in samples. An antibody specific for PRDX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.