Human Peroxiredoxin 4 (PRDX4) ELISA Kit

Peroxiredoxin-4 is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.Sequence analysis predicted that the 271-amino acid protein, 70% identical to a yeast thiol-specific antioxidant, contains a signal peptide and 2 peroxide catalysis cysteine motifs conserved in all human peroxiredoxins. Functional analysis showed that PRDX4 protects glutamine synthetase (GLUL) from inactivation.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Peroxiredoxin 4 (PRDX4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRDX4. No significant cross-reactivity or interference between Human PRDX4 and analogues was observed.

This Human Peroxiredoxin 4 (PRDX4) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX4 in samples. An antibody specific for PRDX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.