Human Prickle-like protein 4 (PRICKLE4) ELISA Kit

C6ORF49 is a member of the LIM domain protein family. C6ORF49 gene contains 9 exons and spans 8.585 kb. By database searching with human PRICKLE1 and PRICKLE2 as probes, Teufel et al. (2005) identified C6ORF49, which they called OEBT, and used this sequence to identify the mouse and rat homologs. The deduced 385-amino acid C6ORF49 protein shares 72% and 54% sequence identity with the mouse and rat homologs, respectively. C6ORF49 contains 2 LIM domains and a PET domain. It is predicted to be localized in the nucleus. EST database analysis revealed C6ORF49 expression in a broad range of normal tissues as well as in hepatocellular carcinoma, breast cancer, and prostate cancer tissues.

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Human Prickle-like protein 4 (PRICKLE4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRICKLE4. No significant cross-reactivity or interference between Human PRICKLE4 and analogues was observed.

This Human Prickle-like protein 4 (PRICKLE4) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE4 in samples. An antibody specific for PRICKLE4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRICKLE4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRICKLE4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRICKLE4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.