Human Proline-rich membrane anchor 1 (PRIMA1) ELISA Kit

As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses. collagen Q (ColQ) anchors AChE at the neuromuscular junction.PRiMA (proline-rich membrane anchor) encoding the AChE anchor in mammalian brain. PRiMA is able to organize AChE into tetramers and to anchor them at the surface of transfected cells. AChE is actually anchored in neural cell membranes through its interaction with PRiMA. PRiMA anchors AChE in mammalian brain and muscle cell membranes. Required to anchor acetylcholinesteras

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Human Proline-rich membrane anchor 1 (PRIMA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRIMA1. No significant cross-reactivity or interference between Human PRIMA1 and analogues was observed.

This Human Proline-rich membrane anchor 1 (PRIMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIMA1 in samples. An antibody specific for PRIMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.