Human CAMP-dependent protein kinase catalytic subunit gamma (PRKACG) ELISA Kit

Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. The PRKACG gene is intronless, contains remnants of a poly (A) tail, is flanked by direct repeats, and is colinear with the PRKACA gene. Thus, the authors concluded that the PRKACG gene is a PRKACA-derived retroposon. Northern blot analysis detected PRKACG expression in fractionated germ cells of human testes.Whereas at the amino acid level C-alpha and C-beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-beta.

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Human CAMP-dependent protein kinase catalytic subunit gamma (PRKACG) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKACG. No significant cross-reactivity or interference between Human PRKACG and analogues was observed.

This Human CAMP-dependent protein kinase catalytic subunit gamma (PRKACG) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACG in samples. An antibody specific for PRKACG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACG bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.