Human Protamine-2 (PRM2) ELISA Kit

Presumably PRM2 is located on human chromosome 16, close to PRM1: in the mouse the corresponding 2 loci are closely linked, and in the Chinese hamster, probes specific for the 2 protamines hybridize to the same restriction fragments after digestion of hamster genomic DNA with any of 5 different restriction endonucleases (Reeves et al., 1987, 1989) . The tight linkage may be of functional significance since PRM1 and PRM2 are among the limited number of genes known to be expressed postmeiotically in haploid cells. In mouse, only one P2 protamine has been detected, whereas in man, the P2 protamine family is represented by 3 protamines, HP2, HP3 and HP4, whose amino acid sequences are identical except at their amino terminal ends .

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Human Protamine-2 (PRM2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRM2. No significant cross-reactivity or interference between Human PRM2 and analogues was observed.

This Human Protamine-2 (PRM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRM2 in samples. An antibody specific for PRM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.