Human Proline-rich protein 1 (PROL1) ELISA Kit

BPLP is a putative 180-amino acid protein of 20.5 kD that contains a 21-residue secretory leader sequence, an N-glycosylation site, and a zona pellucida (Zp) domain. The Zp domain of BPLP shows most similarity to that of ZP2, which carries 2 insertions/deletions of identical size to those in BPLP. The BPLP protein consists of 24.5% proline, 10.5% leucine, and 8.4% each of arginine and serine residues. While the proline residues show 2 regions of clustering, BPLP shows no homology to the salivary proline-rich proteins. BPLP shows limited homology to human PRPb, the mouse MSG proteins, and rat VCS-alpha-1, VCS-beta-1, and submandibular apomucin. Northern blot analysis showed abundant expression of a 1.05-kb BPLP transcript in the lacrimal gland and moderate expression in the submandibular gland.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Proline-rich protein 1 (PROL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PROL1. No significant cross-reactivity or interference between Human PROL1 and analogues was observed.

This Human Proline-rich protein 1 (PROL1) ELISA Kit employs a two-site sandwich ELISA to quantitate PROL1 in samples. An antibody specific for PROL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.