Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit

PRP6 appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. Predicted amino acid sequence of the protein is similar to those of yeast splicing factors, Prp1p/Zer1p of Schizosaccharomyces pombe and Prp6p of Saccharomyces cerevisiae. Among these proteins, tetratrico peptide repeat (TPR) motif, which mediates protein-protein interactions, is conserved, whereas leucine zipper motif is found only in the 100-kDa protein. Indirect immunofluorescent staining showed that the 100-kDa protein localized in the nucleus in HeLa cells.

PRPF6; ANT-1; C20orf14; TOM; U5-102K; hPrp6; PRP6 pre-mRNA processing factor 6 homolog; U5 snRNP-associated 102 kDa protein; androgen receptor N-terminal domain transactivating protein-1; p102 U5 small nuclea

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRPF6. No significant cross-reactivity or interference between Human PRPF6 and analogues was observed.

This Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPF6 in samples. An antibody specific for PRPF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPF6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

PRPF6; ANT-1; C20orf14; TOM; U5-102K; hPrp6; PRP6 pre-mRNA processing factor 6 homolog; U5 snRNP-associated 102 kDa protein; androgen receptor N-terminal domain transactivating protein-1; p102 U5 small nuclea

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.