Human Peripherin-2 (PRPH2) ELISA Kit

Peripherin 2 is a cell surface glycoprotein found in the outer segment of both rod and cone photoreceptor cells. It is located in the rim regions of the flattened disks that contain rhodopsin, which is the protein that is responsible for initiation of visual phototransduction upon reception of light. Peripherin 2 may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. This protein is essential for disk morphogenesis. Defects in this gene are associated with both central and peripheral retinal degenerations. Some of the various phenotypically different disorders are autosomal dominant retinitis pigmentosa, progressive macular degeneration, macular dystrophy and retinitis pigmentosa digenic.

PRPH2; AOFMD; AVMD; CACD2; PRPH; RDS; RP7; TSPAN22; rd2; peripherin 2; peripherin 2; homolog of mouse; peripherin; photoreceptor type; retinal peripherin; tetraspanin-22

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Peripherin-2 (PRPH2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRPH2. No significant cross-reactivity or interference between Human PRPH2 and analogues was observed.

This Human Peripherin-2 (PRPH2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPH2 in samples. An antibody specific for PRPH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

PRPH2; AOFMD; AVMD; CACD2; PRPH; RDS; RP7; TSPAN22; rd2; peripherin 2; peripherin 2; homolog of mouse; peripherin; photoreceptor type; retinal peripherin; tetraspanin-22

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.