Human Thymus-specific serine protease (PRSS16) ELISA Kit

TSSP is thought to play a role in the alternative antigen presenting pathway used by cortical thymic epithelial cells during the positive selection of T cells. The gene is found in the large histone gene cluster on chromosome 6, near the major histocompatibility complex (MHC) class I region. A second transcript variant has been described, but its full length nature has not been determined.The deduced 514-amino acid PRSS16 protein, which the authors termed TSSP, has a calculated molecular mass of 55 kD. PRSS16 contains a putative N-terminal signal peptide and a prolylendopeptidase consensus sequence. Within its 3-prime untranslated region, it has 2 different polyadenylation sites. PRSS16 shares sequence similarity with prolylcarboxypeptidase (PRCP), particularly around the active-site serine.

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Human Thymus-specific serine protease (PRSS16) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS16. No significant cross-reactivity or interference between Human PRSS16 and analogues was observed.

This Human Thymus-specific serine protease (PRSS16) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS16 in samples. An antibody specific for PRSS16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.