Human Testisin (PRSS21) ELISA Kit

PRSS21 encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. It is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and it may be involved in progression of testicular tumors of germ cell origin. The deduced 314-amino acid ESP1 precursor protein has a calculated molecular mass of 33.1 kD, and the 273-amino acid active form has a calculated molecular mass of 30.6 kD. ESP1 contains an N-terminal signal peptide, a propeptide, an active site catalytic triad of conserved his, asp, and ser residues, and a hydrophobic C terminus, which suggests that ESP1 is a membrane-type serine protease.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Testisin (PRSS21) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS21. No significant cross-reactivity or interference between Human PRSS21 and analogues was observed.

This Human Testisin (PRSS21) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS21 in samples. An antibody specific for PRSS21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS21 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.