Human Inactive serine protease 35 (PRSS35) ELISA Kit

Proteolytic degradation of extracellular matrix components has been suggested to play an essential role for the occurrence of ovulation. The plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovulation, are not required in this process. PRSS35, which was upregulated by gonadotropins. PRSS23 was highly expressed in atretic follicles and it was expressed in the ovarian stroma and theca tissues just prior to ovulation. PRSS35 was expressed in the theca layers of developing follicles. It was also highly induced in granulosa cells of preovulatory follicles. PRSS35 was also expressed in the forming and regressing CL. PRSS35 may be involved in ovulation and CL formation and regression, and that PRSS23 may play a role in follicular atresia.

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Human Inactive serine protease 35 (PRSS35) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS35. No significant cross-reactivity or interference between Human PRSS35 and analogues was observed.

This Human Inactive serine protease 35 (PRSS35) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS35 in samples. An antibody specific for PRSS35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS35 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS35 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.