Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD10 encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.The predicted 226-amino acid human p28 protein contains 5 ankyrin repeats, which are thought to function in protein-protein interactions.

PSMD10; RP5-889N15.3; dJ889N15.2; p28; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; ankyrin repeat protein; gankyrin; hepatocellular carcinoma-associated protein p28-I

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMD10. No significant cross-reactivity or interference between Human PSMD10 and analogues was observed.

This Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMD10 in samples. An antibody specific for PSMD10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMD10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMD10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMD10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

PSMD10; RP5-889N15.3; dJ889N15.2; p28; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; ankyrin repeat protein; gankyrin; hepatocellular carcinoma-associated protein p28-I

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.