Human Focal adhesion kinase (FAK) ELISA Kit

PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion and spreading processes. It has been shown that when FAK was blocked, breast cancer cells became less metastastic due to decreased mobility.FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src. This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton.

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Human Focal adhesion kinase (FAK) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTK2. No significant cross-reactivity or interference between Human PTK2 and analogues was observed.

This Human Focal adhesion kinase (FAK) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK2 in samples. An antibody specific for PTK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.