Human Receptor-type tyrosine-protein phosphatase N2 (PTPRN2) ELISA Kit

Receptor-type tyrosine-protein phosphatase N2 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. The catalytic domain of this PTP is most closely related to PTPRN/IA-2beta. This PTP and PTPRN are both found to be major autoantigens associated with insulin-dependent diabetes mellitus. Three alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

PTPRN2; IA-2beta; IAR; ICAAR; PTPRP; IAR/receptor-like protein-tyrosine phosphatase; phogrin; protein tyrosine phosphatase receptor pi; tyrosine phosphatase IA-2 beta

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Receptor-type tyrosine-protein phosphatase N2 (PTPRN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTPRN2. No significant cross-reactivity or interference between Human PTPRN2 and analogues was observed.

This Human Receptor-type tyrosine-protein phosphatase N2 (PTPRN2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPRN2 in samples. An antibody specific for PTPRN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

PTPRN2; IA-2beta; IAR; ICAAR; PTPRP; IAR/receptor-like protein-tyrosine phosphatase; phogrin; protein tyrosine phosphatase receptor pi; tyrosine phosphatase IA-2 beta

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.