Human Securin-2 (PTTG2) ELISA Kit

PTTG2 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. During the course of studies mapping the PTTG1 gene to 5q33, Prezant et al. (1999) found evidence for a related intronless gene, which they referred to as PTTG2, on chromosome 4. They stated that PTTG1 and PTTG2 are highly conserved at both the nucleotide and amino acid levels, including the potential SH3 binding domain. They found low-level PTTG2 expression in normal pituitary and in some other normal tissues, and moderately elevated expression in some pituitary tumors. By radiation hybrid mapping, Prezant et al. (1999) mapped the PTTG2 gene to human chromosome 4p12.

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Human Securin-2 (PTTG2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTTG2. No significant cross-reactivity or interference between Human PTTG2 and analogues was observed.

This Human Securin-2 (PTTG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTTG2 in samples. An antibody specific for PTTG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTTG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTTG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTTG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.