Human Peroxisome biogenesis factor 2 (PXMP3) ELISA Kit

PXMP3 encodes an integral peroxisomal membrane protein required for peroxisome biogenesis. The Peroxisome biogenesis factor 2 is thought to be involved in peroxisomal matrix protein import. Mutations in this gene result in one form of Zellweger syndrome and infantile Refsum disease. Alternative splicing results in multiple transcript variants encoding the same protein.

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Human Peroxisome biogenesis factor 2 (PXMP3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PXMP3. No significant cross-reactivity or interference between Human PXMP3 and analogues was observed.

This Human Peroxisome biogenesis factor 2 (PXMP3) ELISA Kit employs a two-site sandwich ELISA to quantitate PXMP3 in samples. An antibody specific for PXMP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXMP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXMP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXMP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.