Human Peroxisomal membrane protein 4 (PXMP4) ELISA Kit

Pxmp4, which encodes a 24-kDa peroxisomal integral membrane protein of unknown function. Pxmp4 was originally isolated as a 24-kDa polypeptide from rat liver peroxisome membranes (36) . As increased message for Pxmp4 is associated with the NOD allele that confers weaker NKT cell stimulatory capacity, one possibility is that Pxmp4 contributes to the biosynthesis of a nonstimulatory etherphospholipid ligand for CD1d. Pxmp4 may be a component in a fatty acid degradation pathway that catabolizes stimulatory CD1d ligands. Peroxisomes play a critical role in the - and -oxidation of very long chain fatty acids. Pex19 associates with Pxmp4 during or immediately after its translation by free ribosomes. Peroxisomal membrane insertion of the Pxmp4-Pex19 complex is dependent on Pex3p.

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Human Peroxisomal membrane protein 4 (PXMP4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PXMP4. No significant cross-reactivity or interference between Human PXMP4 and analogues was observed.

This Human Peroxisomal membrane protein 4 (PXMP4) ELISA Kit employs a two-site sandwich ELISA to quantitate PXMP4 in samples. An antibody specific for PXMP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXMP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXMP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXMP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.