Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit

ASC2, a PYD-only protein that functions as a modulator of multidomain PYD-containing proteins involved in NF-kappaB and caspase-1 activation. ASC2 adopts a six-helix bundle structure with a prominent loop, comprising 13 amino acid residues, between helices two and three. This loop represents a divergent feature of PYDs from other domains with the DD fold. Detailed analysis of backbone 15N NMR relaxation data using both the Lipari-Szabo model-free and reduced spectral density function formalisms revealed no evidence of contiguous stretches of polypeptide chain with dramatically increased internal motion, except at the extreme N and C termini. Some mobility in the fast, picosecond to nanosecond timescale, was seen in helix 3 and the preceding alpha2-alpha3 loop, in stark contrast to the complete disorder seen in the corresponding region of the NALP1 PYD.

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Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYDC1. No significant cross-reactivity or interference between Human PYDC1 and analogues was observed.

This Human Pyrin domain-containing protein 1 (PYDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYDC1 in samples. An antibody specific for PYDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.