Human Glutamine and serine-rich protein 1 (QSER1) ELISA Kit

QSER1 protein contains two high conserved domains found not only in QSER1 but also in other protein products. This property was conserved from the human QSER1 to the Coelacanth QSER1. Multiple conserved nuclear localization signals were also predicted within the QSER1 protein by pSORT. Predictions of the QSER1 protein structure indicate that the protein contains many alpha helices. NCBI cBLAST predicted structural similarity between the QSER1 protein and the Schizosaccharomyces pombe (fission yeast) RNA Polymerase II A chain. The two regions of similarity occur between amino acids 56-194 and 322-546. This first region (56-194) is a regulatory region in both the human and yeast RNA Polymerase II containing multiple repeats of the sequence YSPTSPSYS.

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Human Glutamine and serine-rich protein 1 (QSER1) ELISA Kit has high sensitivity and excellent specificity for detection of Human QSER1. No significant cross-reactivity or interference between Human QSER1 and analogues was observed.

This Human Glutamine and serine-rich protein 1 (QSER1) ELISA Kit employs a two-site sandwich ELISA to quantitate QSER1 in samples. An antibody specific for QSER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQSER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QSER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QSER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.