Human Sulfhydryl oxidase 1 (QSOX1) ELISA Kit

Sulfhydryl oxidase 1 is a protein that contains domains of thioredoxin and ERV1, members of two long-standing gene families. The gene expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation. Two transcript variants encoding two different isoforms have been found for this gene. Coppock et al. (1993) isolated human embryo lung fibroblast cDNAs that were expressed at a higher level in quiescent cells than in logarithmically growing cells. Several partial cDNAs corresponded to the QSOX1 gene, which the authors called Q6. Northern blot analysis revealed that Q6 is expressed as 3- and 4-kb mRNAs in embryo lung fibroblasts.

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Human Sulfhydryl oxidase 1 (QSOX1) ELISA Kit has high sensitivity and excellent specificity for detection of Human QSOX1. No significant cross-reactivity or interference between Human QSOX1 and analogues was observed.

This Human Sulfhydryl oxidase 1 (QSOX1) ELISA Kit employs a two-site sandwich ELISA to quantitate QSOX1 in samples. An antibody specific for QSOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQSOX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QSOX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QSOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.