Human Ras-related protein Rab-5C (RAB5C) ELISA Kit

Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment. Han et al. (1996) isolated fetal lung cDNAs encoding a RAB protein that they designated RABL (RAB5-like) . The predicted 216-amino acid RABL protein shares 86% identity with RAB5A and RAB5B and contains the conserved GTP-binding site characteristic of RAS proteins. RT-PCR analysis revealed that RABL is expressed ubiquitously. By analysis of cloned segments, Albertsen et al. (1994) determined that the RABL gene, which they called BC1-16, is located in the BRCA1 gene region on chromosome 17q12-q21. Han et al. (1996) refined the map position to 17q21.2 using FISH.

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Human Ras-related protein Rab-5C (RAB5C) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAB5C. No significant cross-reactivity or interference between Human RAB5C and analogues was observed.

This Human Ras-related protein Rab-5C (RAB5C) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB5C in samples. An antibody specific for RAB5C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB5C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB5C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB5C bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.