Human Agrin (AGRN) ELISA Kit

Agrin is a large proteoglycan whose best characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. This protein has nine domains homologous to protease inhibitors. It may also have functions in other tissues and during other stages of development. It is major proteoglycan component in glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.This protein binds to several receptors on the surface of skeletal muscle. The receptor which seems to be required for formation of the neuromuscular junction (NMJ) is called the MuSK receptor (Muscle specific kinase) .

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Agrin (AGRN) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGRN. No significant cross-reactivity or interference between Human AGRN and analogues was observed.

This Human Agrin (AGRN) ELISA Kit employs a two-site sandwich ELISA to quantitate AGRN in samples. An antibody specific for AGRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGRN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGRN bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.