Human Aggrecan core protein (AGC1) ELISA Kit

AGC1 is a member of the aggrecan/versican proteoglycan family. Aggrecan core protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in AGC1 may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in AGC1.

AGC1; AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan 1; large aggregating proteoglycan

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Aggrecan core protein (AGC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGC1. No significant cross-reactivity or interference between Human AGC1 and analogues was observed.

This Human Aggrecan core protein (AGC1) ELISA Kit employs a two-site sandwich ELISA to quantitate AGC1 in samples. An antibody specific for AGC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

AGC1; AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan 1; large aggregating proteoglycan

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.