Human AF4/FMR2 family member 1 (AFF1) ELISA Kit

The sequence of the complete open reading frame for this fusion transcript revealed that the MLL protein is homologous with DNA methyltransferase, the Drosophila trithorax gene product, and the 'AT-hook' motif of high mobility group proteins. An alternative splice that deletes the AT-hook region of MLL was identified. AF4 is a serine- and proline-rich putative transcription factor with a glutamine-rich carboxyl terminus. The composition of the complete MLL-AF4 fusion product argues that it may act through either a gain-of-function or a dominant-negative mechanism in leukemogenesis. This gene is also symbolized MLLT2

AFF1; AF4; MGC134969; MLLT2; PBM1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog; Drosophila) ; translocated to; 2; myeloid/lymphoid or mixed-lineage leukemia trithorax homolog 2; pre-B-cell

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Human AF4/FMR2 family member 1 (AFF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human AFF1. No significant cross-reactivity or interference between Human AFF1 and analogues was observed.

This Human AF4/FMR2 family member 1 (AFF1) ELISA Kit employs a two-site sandwich ELISA to quantitate AFF1 in samples. An antibody specific for AFF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

AFF1; AF4; MGC134969; MLLT2; PBM1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog; Drosophila) ; translocated to; 2; myeloid/lymphoid or mixed-lineage leukemia trithorax homolog 2; pre-B-cell

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.