Human Alpha-2C adrenergic receptor (ADRA2C) ELISA Kit

Alpha-2-adrenergic receptors include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity.ADRa2C encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Alpha-2C adrenergic receptor (ADRA2C) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADRA2C. No significant cross-reactivity or interference between Human ADRA2C and analogues was observed.

This Human Alpha-2C adrenergic receptor (ADRA2C) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRA2C in samples. An antibody specific for ADRA2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADRA2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRA2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRA2C bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.