Human Pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit

Pituitary adenylate cyclase-activating peptide, adenylate cyclase-activating polypeptide 1. Mediated by adenylate cyclase-activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase-activating polypeptide 1 not only is a hypophysiotropic hormone but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene is composed of five exons. Exons 1 and 2 encode the 5' UTR and signal peptide, respectively; exon 4 encodes an adenylate cyclase-activating polypeptide 1-related peptide; and exon 5 encodes the mature peptide and 3' UTR.

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Human Pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADCYAP1. No significant cross-reactivity or interference between Human ADCYAP1 and analogues was observed.

This Human Pituitary adenylate cyclase activating polypeptide (PACAP) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCYAP1 in samples. An antibody specific for ADCYAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCYAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCYAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCYAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.