Human Activin receptor type-2A (ACVR2A) ELISA Kit

ACVR2A is an activin type 2 receptor. This gene encodes activin A type II receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors.

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Human Activin receptor type-2A (ACVR2A) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACVR2A. No significant cross-reactivity or interference between Human ACVR2A and analogues was observed.

This Human Activin receptor type-2A (ACVR2A) ELISA Kit employs a two-site sandwich ELISA to quantitate ACVR2A in samples. An antibody specific for ACVR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACVR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACVR2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACVR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.