Human Alpha-Actinin 3 (ACTN-3) ELISA Kit

Actinin, alpha 3, also known as ACTN3, is a protein which in humans is encoded by the ACTN3 gene.Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments. Skeletal muscle is composed of long cylindrical cells called muscle fibers. There are two types of muscle fibers, slow twitch or muscle contraction (type I) and fast twitch (type II) . Slow twitch fibers are more efficient in using oxygen to generate energy whilst fast twitch fibers are less efficient. However, fast twitch fibers fire more rapidly and generate more force.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Alpha-Actinin 3 (ACTN-3) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTN3. No significant cross-reactivity or interference between Human ACTN3 and analogues was observed.

This Human Alpha-Actinin 3 (ACTN-3) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTN3 in samples. An antibody specific for ACTN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.